Cryogenic Operation of sCMOS Image Sensors

Scientific CMOS image sensors have lower read noise and dark current than charge coupled devices. They are also uniquely qualified for operation at cryogenic temperatures due to their MOSFET pixel architecture. This paper follows the design of a cryogenic imaging system to be used as a star tracking rocket attitude regulation system. The detector was proven to retain almost all its sensitivity at cryogenic temperatures with acceptably low read noise. Once the star tracker successfully maintains rocket attitude during the flight of the CIBER-2 experiment, the technology readiness level of scientific CMOS detectors will advance enough that they could see potential applications in deep space imaging experiments

Holocene-Neogene volcanism in northeastern Australia: chronology and eruption history

Quaternary and late Neogene volcanism is widespread in northeastern Australia, producing at least 397 eruptions covering more than 20,000 km2, including at least 20 flows over 50 km long. Despite this abundance of young volcanism, before this study numerous eruptions had tentative ages or were undated, and the area requires a comprehensive evaluation of eruption patterns through time. To help address these issues we applied multi-collector ARGUS-V 40Ar/39Ar geochronology to determine the age of four of the younger extensive flows: Undara (160 km long, 189 ± 4/4 ka; 2σ, with full analytical/external uncertainties), Murronga (40 km long, 153 ± 5/5 ka), Toomba (120 km long, 21 ± 3/3 ka), and Kinrara (55 km long, 7 ± 2/2 ka). Verbal traditions of the Gugu Badhun Aboriginal people contain features that may potentially describe the eruption of Kinrara. If the traditions do record this eruption, they would have been passed down for 230 ± 70 generations – a period of time exceeding the earliest written historical records. To further examine north Queensland volcanism through time we compiled a database of 337 ages, including 179 previously unpublished K-Ar and radiocarbon results. The compiled ages demonstrate that volcanic activity has occurred without major time breaks since at least 9 Ma. The greatest frequency of eruptions occurred in the last 2 Ma, with an average recurrence interval of <10–22 ka between eruptions. Activity was at times likely more frequent than these calculations indicate, as the geochronologic dataset is incomplete, with undated eruptions, and intraplate volcanism is often episodic. The duration, frequency, and youthfulness of activity indicate that north Queensland volcanism should be considered as potentially still active, and there are now two confirmed areas of Holocene volcanism in eastern Australia – one at each end of the continent. More broadly, our data provides another example of 40Ar/39Ar geochronology applied to Holocene and latest Pleistocene mafic eruptions, further demonstrating that this method has the ability to examine eruptions and hazards at the youngest volcanoes on Earth

Structural transitions, octahedral rotations, and electronic properties of A3A_3Ni2_2O7_7 rare-earth nickelates under high pressure

Motivated by the recent observation of superconductivity with $T_c \sim 80$ K in pressurized La3Ni2O7 [Nature 621, 493 (2023)], we explore the structural and electronic properties in A3Ni2O7 bilayer nickelates (A=La-Lu, Y, Sc) as a function of hydrostatic pressure (0-150 GPa) from first principles including a Coulomb repulsion term. At $\sim 20$ GPa, we observe an orthorhombic-to-tetragonal transition in La$_3$Ni$_2$O$_7$ at variance with recent x-ray diffraction data, which points to so-far unresolved complexities at the onset of superconductivity, e.g., charge doping by variations in the oxygen stoichiometry. We compile a structural phase diagram with particular emphasis on the $b/a$ ratio, octahedral anisotropy, and octahedral rotations. Intriguingly, chemical and external pressure emerge as two distinct and counteracting control parameters. We find unexpected correlations between $T_c$ and the in-plane Ni-O-Ni bond angles for La$_3$Ni$_2$O$_7$. Moreover, two novel structural phases with significant $c^+$ octahedral rotations and in-plane bond disproportionations are uncovered for A=Nd-Lu, Y, Sc that exhibit a surprising pressure-driven electronic reconstruction in the Ni $e_g$ manifold. By disentangling the involvement of basal versus apical oxygen states at the Fermi surface, we identify Tb$_3$Ni$_2$O$_7$ as an interesting candidate for superconductivity at ambient pressure. These results suggest a profound tunability of the structural and electronic phases in this novel materials class and are key for a fundamental understanding of the superconductivity mechanism.Comment: 12 pages, 5 figure

Impact demagnetization of the Martian crust: Current knowledge and future directions

The paleomagnetism of the Martian crust has important implications for the history of the dynamo, the intensity of the ancient magnetic field, and the composition of the crust. Modification of crustal magnetization by impact cratering is evident from the observed lack of a measurable crustal field (at spacecraft altitude) within the youngest large impact basins (e.g., Hellas, Argyre and Isidis). It is hoped that comparisons of the magnetic intensity over impact structures, forward modeling of subsurface magnetization, and experimental results of pressure-induced demagnetization of rocks and minerals will provide constraints on the primary magnetic mineralogy in the Martian crust. Such an effort requires: (i) accurate knowledge of the spatial distribution of the shock pressures around impact basins, (ii) crustal magnetic intensity maps of adequate resolution over impact structures, and (iii) determination of demagnetization properties for individual rocks and minerals under compression. In this work, we evaluate the current understanding of these three conditions and compile the available experimental pressure demagnetization data on samples bearing (titano-) magnetite, (titano-) hematite, and pyrrhotite. We find that all samples demagnetize substantially at pressures of a few GPa and that the available data support significant modification of the crustal magnetic field from both large and small impact events. However, the amount of demagnetization with applied pressure does not vary significantly among the possible carrier phases. Therefore, the presence of individual mineral phases on Mars cannot be determined from azimuthally averaged demagnetization profiles over impact basins at present. The identification of magnetic mineralogy on Mars will require more data on pressure demagnetization of thermoremanent magnetization and forward modeling of the crustal field subject to a range of plausible initial field and demagnetization patterns.United States. National Aeronautics and Space Administration (NNG04GD17G)United States. National Aeronautics and Space Administration (NNX07AQ69G)United States. National Aeronautics and Space Administration (NNX06AD14G

Ethanol-induced Modulation of Hepatocellular Extracellular Signal-regulated Kinase-1/2 Activity via 4-Hydroxynonenal

Modulation of the extracellular signal-regulated kinases (ERK-1/2), a signaling pathway directly associated with cell proliferation, survival, and homeostasis, has been implicated in several pathologies, including alcoholic liver disease. However, the underlying mechanism of ethanol-induced ERK-1/2 modulation remains unknown. This investigation explored the effects of ethanol-associated oxidative stress on constitutive hepatic ERK-1/2 activity and assessed the contribution of the lipid peroxidation product 4-hydroxynonenal (4-HNE) to the observations made in vivo. Constitutive ERK-1/2 phosphorylation was suppressed in hepatocytes isolated from rats chronically consuming ethanol for 45 days. This observation was associated with an increase in 4-HNE-ERK monomer adduct concentration and a hepatic cellular and lobular redistribution of ERK-1/2 that correlated with 4-HNE-protein adduct accumulation. Chronic ethanol consumption was also associated with a decrease in hepatocyte nuclear ELK-1 phosphorylation, independent of changes in total nuclear ELK-1 protein. Primary hepatocytes treated with concentrations of 4-HNE consistent with those occurring during oxidative stress displayed a concentration-dependent decrease in constitutive ERK-1/2 phosphorylation, activity, and nuclear localization that negatively correlated with 4-HNE-ERK-1/2 monomer adduct accumulation. These data paralleled the decreased phosphorylation of the downstream kinase ELK-1. Molar ratios of purified ERK-2 to 4-HNE consistent with pathologic ratios found in vivo resulted in protein monomer-adduct formation across a range of concentrations. Collectively, these data demonstrate a novel association between ethanol-induced lipid peroxidation and the inhibition of constitutive ERK-1/2, and suggest an inhibitory mechanism mediated by the lipid peroxidation product 4-hydroxynonenal

Ultrasound capsule endoscopy:sounding out the future

Video capsule endoscopy (VCE) has been of immense benefit in the diagnosis and management of gastrointestinal (GI) disorders since its introduction in 2001. However, it suffers from a number of well recognized deficiencies. Amongst these is the limited capability of white light imaging, which is restricted to analysis of the mucosal surface. Current capsule endoscopes are dependent on visual manifestation of disease and limited in regards to transmural imaging and detection of deeper pathology. Ultrasound capsule endoscopy (USCE) has the potential to overcome surface only imaging and provide transmural scans of the GI tract. The integration of high frequency microultrasound (µUS) into capsule endoscopy would allow high resolution transmural images and provide a means of both qualitative and quantitative assessment of the bowel wall. Quantitative ultrasound (QUS) can provide data in an objective and measurable manner, potentially reducing lengthy interpretation times by incorporation into an automated diagnostic process. The research described here is focused on the development of USCE and other complementary diagnostic and therapeutic modalities. Presently investigations have entered a preclinical phase with laboratory investigations running concurrently

Human Dna2 is a nuclear and mitochondrial DNA maintenance protein

Dna2 is a highly conserved helicase/nuclease that in yeast participates in Okazaki fragment processing, DNA repair, and telomere maintenance. Here, we investigated the biological function of human Dna2 (hDna2). Immunofluorescence and biochemical fractionation studies demonstrated that hDna2 was present in both the nucleus and the mitochondria. Analysis of mitochondrial hDna2 revealed that it colocalized with a subfraction of DNA-containing mitochondrial nucleoids in unperturbed cells. Upon the expression of disease-associated mutant forms of the mitochondrial Twinkle helicase which induce DNA replication pausing/stalling, hDna2 accumulated within nucleoids. RNA interference-mediated depletion of hDna2 led to a modest decrease in mitochondrial DNA replication intermediates and inefficient repair of damaged mitochondrial DNA. Importantly, hDna2 depletion also resulted in the appearance of aneuploid cells and the formation of internuclear chromatin bridges, indicating that nuclear hDna2 plays a role in genomic DNA stability. Together, our data indicate that hDna2 is similar to its yeast counterpart and is a new addition to the growing list of proteins that participate in both nuclear and mitochondrial DNA maintenance

Computational Ranking of Yerba Mate Small Molecules Based on Their Predicted Contribution to Antibacterial Activity against Methicillin-Resistant Staphylococcus aureus

The aqueous extract of yerba mate, a South American tea beverage made from Ilex paraguariensis leaves, has demonstrated bactericidal and inhibitory activity against bacterial pathogens, including methicillin-resistant Staphylococcus aureus (MRSA). The gas chromatography-mass spectrometry (GC-MS) analysis of two unique fractions of yerba mate aqueous extract revealed 8 identifiable small molecules in those fractions with antimicrobial activity. For a more comprehensive analysis, a data analysis pipeline was assembled to prioritize compounds for antimicrobial testing against both MRSA and methicillin-sensitive S.aureus using forty-two unique fractions of the tea extract that were generated in duplicate, assayed for activity, and analyzed with GC-MS. As validation of our automated analysis, we checked our predicted active compounds for activity in literature references and used authentic standards to test for antimicrobial activity. 3,4-dihydroxybenzaldehyde showed the most antibacterial activity against MRSA at low concentrations in our bioassays. In addition, quinic acid and quercetin were identified using random forests analysis and 5-hydroxy pipecolic acid was identified using linear discriminant analysis. We also generated a ranked list of unidentified compounds that may contribute to the antimicrobial activity of yerba mate against MRSA. Here we utilized GC-MS data to implement an automated analysis that resulted in a ranked list of compounds that likely contribute to the antimicrobial activity of aqueous yerba mate extract against MRSA

Design and Implementation of a Studio-Based General Chemistry Course

Most students taking general chemistry courses do not intend to pursue careers in chemistry; in fact, they are more likely to end up in positions where they fund, write, or vote for chemical research and policies. Our profession continues to ask how we can teach students scientific reasoning skills and chemical understanding in general chemistry that they are able to take beyond the classroom into their everyday lives. The emerging answer at this university is the studio teaching method, which incorporates the “best teaching and learning practices†recommended by chemical education research within an integrated lecture–lab technology-intensive environment. The design, implementation, and pedagogical rationale of studio general chemistry are described

Ultrasound mediated delivery of quantum dots from a proof of concept capsule endoscope to the gastrointestinal wall

Biologic drugs, defined as therapeutic agents produced from or containing components of a living organism, are of growing importance to the pharmaceutical industry. Though oral delivery of medicine is convenient, biologics require invasive injections because of their poor bioavailability via oral routes. Delivery of biologics to the small intestine using electronic delivery with devices that are similar to capsule endoscopes is a promising means of overcoming this limitation and does not require reformulation of the therapeutic agent. The efficacy of such capsule devices for drug delivery could be further improved by increasing the permeability of the intestinal tract lining with an integrated ultrasound transducer to increase uptake. This paper describes a novel proof of concept capsule device capable of electronic application of focused ultrasound and delivery of therapeutic agents. Fluorescent markers, which were chosen as a model drug, were used to demonstrate in vivo delivery in the porcine small intestine with this capsule. We show that the fluorescent markers can penetrate the mucus layer of the small intestine at low acoustic powers when combining microbubbles with focused ultrasound during in vivo experiments using porcine models. This study illustrates how such a device could be potentially used for gastrointestinal drug delivery and the challenges to be overcome before focused ultrasound and microbubbles could be used with this device for the oral delivery of biologic therapeutics